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ATCC
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JCRB Cell Bank
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BioResource International Inc
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Blackwell Science Ltd
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Millipore
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Santa Cruz Biotechnology
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European Collection of Authenticated Cell Cultures
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Eurofins
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China Center for Type Culture Collection
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Thermo Fisher
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Lonza
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Merck KGaA
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Image Search Results
Journal: The Journal of Cell Biology
Article Title: Ip-10 Inhibits Epidermal Growth Factor–Induced Motility by Decreasing Epidermal Growth Factor Receptor–Mediated Calpain Activity
doi:
Figure Lengend Snippet: Hs68 Cellular Morphometry
Article Snippet:
Techniques:
Journal: Oncotarget
Article Title: Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity
doi: 10.18632/oncotarget.6488
Figure Lengend Snippet: A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and Hs68 cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.
Article Snippet: The normal
Techniques: Derivative Assay, cDNA Library Assay, Clone Assay, Transfection, Western Blot, Expressing, Staining, Membrane, Control, Double Immunofluorescence Staining, Fluorescence, Microscopy